The growth. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. 6. TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate Spectrophotometer,andVersaMax™MicroplateSpectrophotometerproviderapidandsensitive. Integration delay. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. ZERO BIAS - scores, article reviews, protocol conditions and moreAbsorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. Buffer (100 mmol L –1acetate buffer, pH 5. D. The GH Pro 340 Supplemental Greenhouse LED grow lighting is designed with greenhouse operations in mind. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). SpectraMax Multi-Mode Microplate Readers. SPECTRAmax® 190. MOLECULAR DEVICE M5. Details. Spectramax 340pc User Manual, Hudson River Saranac Pellet Stove Manual, Alesis Midiverb 1 Manual, User Manual For Samsung Galaxy Ace Duos S6802, Di Style Manual, R150f Transmission Repair Manual, Eos5d Mark 2 Manual. , Sunnyvale, CA). When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 001/min at 425 nm. SpectraMax QuickDrop Micro-Volume Spectrophotometer. TGFα release was calculated as described in the Results. The contents of the. Fibrin formation was measured by turbidity at 405 nm using a SpectraMax 340 PC plate reader. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. The OD 490 was measured with an absorbance microplate reader (SpectraMax 340; Molecular Devices). mums. or microplate reader (e. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). SpectraMax® Microplate Readers and SoftMax® Pro Software. 20. SoftMax Pro. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). LBA Formats for Pharmacokinetic AssaysAfter treatment, 10 μl of CCK-8 was added to each well, and the incubation was continued at 37°C for 2 h in the dark. Molecular Devices SpectraMax M5e Microplate Reader. Background: A computerized machine, SPECTRAmax 340, was used to evaluate the recently reported phenomenon of antagonism of the polyene amphotericin B (AMB) in Candida albicans pre-exposed to the triazole fluconazole (FLZ). SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Furthermore, the stain was extracted using 10% acetic acid, and its absorbance was measured at 595 nm in a microtiter plate reader (SpectraMax 340; Molecular Devices). #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). ISM Equipment Classification (Group 1, Class A) This equipment is designated as. 30 µs. Endpoint, kinetic, spectrum. Briefly, ear or dorsal skin was dissected and fixed with 10% phosphate buffered formalin for 24–72 h. Alves, Amelia K. 5 ng/ml; estradiol: 10 pg/ml. 304 signal level saturation. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. After washing, the dye was eluted with 3% HCl, and the absorbance measured with a SpectraMax 340 microtiter plates reader at 665 nm. The VersaMax and SpectraMax 190 read 96-well microplates. One plate carrier adapter is provided with the instrument. 0. 5 hours, and is measured in a 96-well plate spectrophotometer at 405 nm (SpectraMax 340; Molecular Devices LLC, Sunnyvale, CA, USA). Calculating % of total mediator release in RBL assay. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. edu Contact Phone: 216-368-8962. Immunofluorescence and Aggregation Analysis. 003 OD Stray light ≤ 0. 6 g sodium acetate trihydrate and 2. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The extreme flexibility and high sensitivity of the SpectraMax GEMINI EM make it appropriate for applications within the fields of bio-A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. Filterless readers provide consistent results with steady temperature regulation. Wells were washed three times. 2. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Cell culture 3T3 fibroblasts and RAW 264. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San Jose, CA, USA). LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). SpectraMax Multi-Mode Microplate Readers. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Filipin StainingSpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. MMP‑1 inhibition assay. #PLUS 384) MethodsSpectraMax 340 PC, Molecular Devices, USA was used as a 96-well microplate reader to test bioactivities. Labsystems Luminoskan RT Microplate Reader. Create custom fluorescence assays without having to purchase expensive filter sets. 81. Plasma glucose levels were measured using FreeStyle Lite (Abbott Diabetes Care Ltd. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax Gemini XS Microplate Fluorometer $2,800. 0, MSD) or Spectramax 340 (Molecular Devices). Molecular Devices SpectraMax M5e Microplate Reader $26,500. Customer Transformation Hub. Protein electrophoresis system (BioRad, Hercules, CA) and Odyssey Infrared Imaging System (Li-Cor Biosciences, Lincoln, NE) were used in western blot. using SpectraMax i3 (Molecular devices, San Jose, USA). Or scan up to 384 wells at any time to determine the best assay conditions. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Related Products: SpectraMax 340 Absorbance Microplate Reader, Molecular Devices SpectraMax 384 Plus UV/Vis Microplate Reader, Molecular DevicesGETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS applications guide to microplate systems applications guide to microplate systems450 nm using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). MCE has not independently confirmed the accuracy of these methods. 6) and 0. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. The levels of rheumatoid factor (RF) antibodies were measured at the Clinical Immunology Laboratory of the Sahlgrenska University Hospital or Leiden Clinical Immunology Laboratory. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature. , Sunnyvale, CA). ELISA plates were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Samples were considered “positive” if the absorbance value was greater than two times the average absorbance value of the negative control wells . The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. A 340 μL of 50 mM borate buffer (pH 9), 240 μL methanol, 10 μL standard solution or sample, and 20 μL of 0. The SPECTRAmax 340PC can measure. SpectraMax 190 reads 96-well and strip-well plates. Molecular Device SpectraMax M3 Plate Reader - Certified with Warranty. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Samples with OD values of less than 0. 04. After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. 5, 2 mM pNPP, and recombinant enzymes, PTP1B activities were continuously monitored on a SpectraMax 340 Microplate Reader (SpectraMax, Silicon Valley, CA, USA) at 405 nm for 2 min at 30°C. Accurate quantitation The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. The SPECTRAmax 340PC can mea- TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes acrossawiderangeofconcentrations. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate ReaderThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. 2. TDP-43 stress granular cells were treated with 70% ice-cold. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). MSD read buffer and TMB one component HRP substrate are from MSD and SurModics (Eden Prairile, MN), respectively. ). Table 1. The adapter is. Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Assay setup for a 384-well low-volume plate. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). The metals were preincubated with A for 5 min when added before heme. The cells were pretreated, serum starved for 24 h, and then treated with NK-cdM priora SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). We also offer depot Repair Service from our base of operations in Southern California (San Diego). The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. 5. Immunofluorescence and Aggregation Analysis. 6) and 0. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). These isotypes cause antigen cross-linking by multimeric binding and self. The cell survival. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. 4. A 0. a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). The ABS reader ranges from. Product Keyword. Near bottom. Samples containing 25 μg of protein were separated on 12. Products : SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax 250 (Obsolete), SpectraMax 340 (Obsolete), VersaMax reader How does stray light affect my OD reading? The basic problem caused by stray light (light you don't want, getting into the read) is a falsely low OD seen. This reader only allows high performance HTRF measurement when assays are run in. Not all manufacturers' microplates are the same with regard to design, materials, orAbsorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). The cell proliferation on different Ti discs was assessed by measuring the absorbance at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Devices, Sunnyvale, CA). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the. Measurements of OD at 540 nm were then performed in a microtiter plate reader (SpectraMax 340 microplate reader; Molecular Devices Corp. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. , Sunnyvale, CA, USA) at 595 nm. ) at 490 nm. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. Hydrogen peroxide (H 2 O 2) was used to establish an intracellular oxidative. Assay buffer was 50 m M Hepes, pH 7. One plate carrier adapter is provided with the instrument. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Acceptor filter: 665 nm. These robust readers have been placed in labs from Antarctica to the. 1% N-1-naphtylethylenediamine dihydrochloride, 2. Donor: 620 nm. albicans pre. Also for: Spectramax 190, Spectramax 340pc384, Versamax. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. The SpectraMax 340PC384 Microplate reader supports various absorbance assay categories, such as protein quantitation, endotoxin detection, microbial growth, ELISAs. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Stat Fax 3200 Microplate Reader. . Absorbance was measured at 490/650 nm by using a SpectraMax 340 microplate spectrophotometer® (Molecular Devices, Sunnyvale, CA, U. TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027 EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm EX laser diode and a 570 nm (100) EM filter Pick best speed, sensitivity, and price for your needs Table 1. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Each SpectraMax® 340 PC 384 Microplate Reader has an. Following incubation, 100 μl of solution was transferred to new wells and the color change in the solution was measured with a microtiter plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. 1% N-1-naphtylethylenediamine dihydrochloride, 2. Absorbance was measured at 450 nm by using a SpectraMax 340 microplate spectrophotometer (Molecular Device). Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Each experiment was performed in duplicate and repeated at. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. SpectraMax Multi-Mode Microplate Readers. 5. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). #ABS PLUS) Methods. Supplier: Molecular Devices. All assays were performed using a sample size of 12, and the absorbance at 425 nm was measured by using a SpectraMax 340 multiwell plate reader (Molecular Devices, Sunnyvale, CA). They are for reference only. All data were normalized to experimental day 0. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. Products : SpectraMax Plus 384 reader,. SpectraMax Injector Cartridge with SmartInject™ Technology. Data System. Changes in OD 405 nm were followed on the SpectraMAX 340 plate reader. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Upgradeable modules are also available including western blot, cell imaging, and fast. Revvity. 0200-6117. Service parts for these models are not being produced and are no longer in stock. SOD activities were calculated from assays in which there was an inhibition of cytochrome c reduction of between 40 and 50%. A. Briefly, cells were sequentially treated with terminal deoxynucleotidyl. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. It includes both a lamp and a new lamp holder. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. SpectraMax Multi-Mode Microplate Readers. Plates coated with no antigen, canine CD19, streptavidin alone, and streptavidin with irrelevant human avitag-CD3ε (Acro Biosystems, Newark, DE) were used as negative controls. All absorbance measurements were taken on a SpectraMax 340 PC reader equipped with SoFTMax Pro software. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices). The reaction was then stopped by adding 50 µL of 1 M sulfuric acid to each well. Details. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). Values were determined according to a standard curve (log/log curve-fit). 306 plate air cal. CD154 is an established indicator of antigen-reactive T helper cells in other species, but has not been characterized in horses. Quick View. 0. 3. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Call 1-800-745-2710. , Sunnyvale, CA, USA) at a wavelength of 450 nm. Fig. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. 314 low lamp level warning. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 0, 20 mmol L –1cal-cium chloride). 5 mL sample or standard solution was well mixed with 0. RESEARCH ARTICLE Effects of early-life conditions on innate immune function in adult zebra finches Merijn M. The possi-ble effect of zinc or copper on the binding of heme by A was tested as described above by using a 50 and 100 M final concentration of each metal. Subsequent replacements require only the lamp (N-34-LB) be replaced. SpectraMax 190 reads 96-well and strip-well plates. On 1, 7, 14, and 21 days, the cells were washed with DPBS twice and incubated with CCK-8 solution for 2 h in the dark at 37 °C. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. Details. The SpectraMax GEMINI EM cannot be operated without the computer and SoftMax Pro software. 340 nm / 70 nm. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 2 0. Each experiment was performed in duplicate. The plates were incubated for 4 additional hours at 37°C. SPECTRAmax® 190. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). Molecular Devices. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. The reaction mixture was incubated for 5 min. 1 mL of acetylacetone. Developed plates were read on MSD reader Sector Imager S 600, Model 1200, with Discovery Workbench Software (version 4. 4. ) to the upper chamber, followed by measurement with a spectrophotometer (SpectraMax 340, Molecular Devices LLC, Sunnyvale, CA, USA) at 610 nm of media in the lower chamber after one hour . Microplate Reader Shelf. 2 M DEEMM were mixed in a microtube,. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. 5 mL of 1 M acetate buffer (pH 4. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hFollowing incubation, absorbance was immediately measured at 405 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, LLC. 1 mM MnCl2 and 25 mM CHES buffer, pH 9. One plate carrier adapter is provided with the instrument. SPECTRAMAX 340. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. Recalcification (clotting) time was determined by monitoring absorbance at 650 nm at 10-sec intervals in a SpectraMax 340 microtiter plate reader, with onset time (the time to a linear increase in the OD, which reflects the maximal rate of formation of insoluble fibrin) set at an OD of 0. The ability of PTK787/ZK 222584 to inhibit angiogenesis was tested in an in vitro model of capillary sprout formation (64) . application or budget. Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). Data are expressed as percent of maximum binding for each protein. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)Bob - 06 Apr. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. 10. 190-1000 nm, tunable in 1 nm increments. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. 190-1000 nm. The. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Molecular Devices Corporation 1311. 340-850 nm: Wavelength Selection: Monochromator, turnable in 1 nm increments:. The antibodies originally defining this molecule by inducing apoptosis, anti-Fas and anti-APO-1 , were of the IgM or IgG3 subtype, respectively. 5. Molecular Devices Spectramax 340 Plate Reader Repair. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. Not. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. Software (SoftMax Pro ® software or comparable plate reader software). #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Like CD3, the CD95 death receptor requires binding of immobilized or multimeric antibodies to be activated effectively. Data were expressed as the mean percent viable cell vs. used in the SPECTRAmax PLUS. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. Microplate Readers. Wavelength range: 340-850 nm. , Sunnyvale CA). The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Values are expressed as the mean s ± SD of 3 separate experiments, each performed in triplicate. 5% phosphoric acid) in microplates at room temperature for 10 min. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. #N207) LAL Reagent Water (Lonza cat. These models can no longer be repaired. Column chromatography was performed on silica gel (Kieselgel 60, 70–230 mesh, Merck). Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. Intra- and inter-assay coefficients of variation were lower than 8. The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. Oxidative stress induction by H 2 O. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. Statistical AnalysisConsult Molecular Devices's entire SpectraMax i3x catalogue on MedicalExpo. 1. 0 2. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. Immunofluorescence and Aggregation Analysis. Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. A standard curve was constructed by sequential dilution of a TNFα standard from 2000-15 pg/mL. Subsequently, the mixture was heated at 100 °C for 10. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. Irene Tieleman, Ido PenandSimon VerhulstSubsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Briefly, cells were seeded into a. 5. 25–0. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. Manufacturer: Molecular Devices Model: SpectraMax The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. The first time you replace an OEM lamp you must use the N-34-MLA. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). StakMax. Call 1-800-745-2710. Description Control Panel Cuvette Chamber Back Panel Microplate Drawer The Control Panel TEMP on/off TEMP Figure 1. Molecular Devices SPECTRAmax. 6. Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. Microplate Spectrophotometer Operator’s Manual. SpectraMAX 340. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. SpectraMax Multi-Mode Microplate Readers. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Sandwich ELISAs were used to measure insulin (DY8056, R&D Systems, Minneapolis, MN, USA). The manufacturer of the SpectraMax 340PC Microplate Spectrophotometer is Molecular Devices. 313 reference gain check fail. Operators can conveniently select up to 6 wavelengths at a time for. Molecular Devices SpectraMax Gemini XS Microplate Reader. The values were corrected for nonspecific background by subtracting the OD 650. 1% Chaps, 10 m M dithiothreitol, and 10% sucrose. For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The values were compared against a standard curve that was generated using known concentrations of IL6 to calculate concentration in the. Samples were analyzed in triplicates. Not all manufacturers' microplates are the same with regard to design, materials, orSpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. The reduction of HED in the presence of NADPH and GR was recorded at 340 nm. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 1% PEG 8000 at 37°C. About GMI provides new and GMI Certified Pre-Owned scientific instrumentation and service to research universities, environmental labs, forensic investigators, biotechnology and pharmaceutical companies, hospitals, clinics and analytical laboratories, in the United. Awareness Technology, Inc. Key benefits • User-upgradeable application modules including cellular imaging SpectraTest ABS1 Absorbance Validation Plate. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). , Sunnyvale, CA) at 490 nm. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. A 0. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. Features showcased by this model include. TDP-43 stress granular cells were treated with 70% ice-cold. 4) was followed according to Vanacker et al. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. #N207) LAL Reagent Water (Lonza cat.